The cryo-EM method microcrystal electron diffraction (MicroED).
review · Level V
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- Record sourced from PubMed, PMID 31040436.
- Also identified by DOI 10.1038/s41592-019-0395-x and PMC identifier 6568260.
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Abstract
In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal electron diffraction (MicroED). Since that time, data collection and analysis schemes have been fine-tuned, and structures for more than 40 different proteins, oligopeptides and organic molecules have been determined. Here we review the MicroED technique and place it in context with other structure-determination methods. We showcase example structures solved by MicroED and provide practical advice to prospective users.
Medical subject headings
- Cryoelectron Microscopy
- Crystallography, X-Ray
- Microscopy, Electron, Transmission
- Proteins