Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31061492.
- Also identified by DOI 10.1038/s41592-019-0401-3 and PMC identifier 6557432.
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Abstract
We designed an epi-illumination SPIM system that uses a single objective and has a sample interface identical to that of an inverted fluorescence microscope with no additional reflection elements. It achieves subcellular resolution and single-molecule sensitivity, and is compatible with common biological sample holders, including multi-well plates. We demonstrated multicolor fast volumetric imaging, single-molecule localization microscopy, parallel imaging of 16 cell lines and parallel recording of cellular responses to perturbations.
Medical subject headings
- Drosophila
- Image Processing, Computer-Assisted
- Imaging, Three-Dimensional
- Lighting
- Microscopy, Fluorescence
- Molecular Imaging
- Single-Cell Analysis