Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31086181.
- Also identified by DOI 10.1038/s41467-019-09910-7 and PMC identifier 6513864.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery. Major challenges often arise from the vast structural diversity of glycans in combination with limited sample amounts. Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans. This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.
Medical subject headings
- Glycomics
- Polysaccharides
- Spectrometry, Mass, Electrospray Ionization
- Tandem Mass Spectrometry