Construction of a pathway to C50-ε-carotene.
basic_science · Level V
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- Record sourced from PubMed, PMID 31086389.
- Also identified by DOI 10.1371/journal.pone.0216729 and PMC identifier 6516660.
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Abstract
Substrate tolerance of bacterial cyclases has been demonstrated in various contexts, but little is known about that of plant cyclases. Here, we tested two plant ε-cyclases to convert C50-lycopene, which we previously established by rounds of directed evolution. Unlike bacterial β-cyclases, two-end cyclase from lettuce exhibited complete specificity against this molecule, indicating that this enzyme has some mechanism that exerts size-specificity. Arabidopsis one-end cyclase At-y2 showed detectable activity to C50-lycopene. Interestingly, we found that it functions as a two-end cyclase in a C50 context. Based on this observation, a possible model for substrate discrimination of this enzyme is proposed.
Medical subject headings
- Carotenoids
- Lyases
- Metabolic Engineering