HiChIRP reveals RNA-associated chromosome conformation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31133759.
- Also identified by DOI 10.1038/s41592-019-0407-x and PMC identifier 6638558.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Modular domains of long non-coding RNAs can serve as scaffolds to bring distant regions of the linear genome into spatial proximity. Here, we present HiChIRP, a method leveraging bio-orthogonal chemistry and optimized chromosome conformation capture conditions, which enables interrogation of chromatin architecture focused around a specific RNA of interest down to approximately ten copies per cell. HiChIRP of three nuclear RNAs reveals insights into promoter interactions (7SK), telomere biology (telomerase RNA component) and inflammatory gene regulation (lincRNA-EPS).
Medical subject headings
- Chromatin
- Embryonic Stem Cells
- Gene Expression Regulation
- RNA
- RNA, Long Noncoding
- Telomerase