Blimp1<sup>+</sup> cells generate functional mouse sebaceous gland organoids in vitro.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31138796.
- Also identified by DOI 10.1038/s41467-019-10261-6 and PMC identifier 6538623.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Most studies on the skin focus primarily on the hair follicle and interfollicular epidermis, whereas little is known regarding the homeostasis of the sebaceous gland (SG). The SG has been proposed to be replenished by different pools of hair follicle stem cells and cells that resides in the SG base, marked by Blimp1. Here, we demonstrate that single Blimp1<sup>+</sup> cells isolated from mice have the potential to generate SG organoids in vitro. Mimicking SG homeostasis, the outer layer of these organoids is composed of proliferating cells that migrate inward, undergo terminal differentiation and generating lipid-filled sebocytes. Performing confocal microscopy and mass-spectrometry, we report that these organoids exhibit known markers and a lipidomic profile similar to SGs in vivo. Furthermore, we identify a role for c-Myc in sebocyte proliferation and differentiation, and determine that SG organoids can serve as a platform for studying initial stages of acne vulgaris, making this a useful platform to identify potential therapeutic targets.
Medical subject headings
- Cell Differentiation
- Cell Proliferation
- Organoids
- Positive Regulatory Domain I-Binding Factor 1
- Proto-Oncogene Proteins c-myc
- Sebaceous Glands