Direct wavefront sensing enables functional imaging of infragranular axons and spines.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31209383.
- Also identified by DOI 10.1038/s41592-019-0434-7 and PMC identifier 6763395.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We advance two-photon microscopy for near-diffraction-limited imaging up to 850 µm below the pia in awake mice. Our approach combines direct wavefront sensing of light from a guidestar (formed by descanned fluorescence from Cy5.5-conjugated dextran in brain microvessels) with adaptive optics to compensate for tissue-induced aberrations in the wavefront. We achieve high signal-to-noise ratios in recordings of glutamate release from thalamocortical axons and calcium transients in spines of layer 5b basal dendrites during active tactile sensing.
Medical subject headings
- Axons
- Dendritic Spines