Imaging neuropeptide release at synapses with a genetically engineered reporter.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31241464.
- Also identified by DOI 10.7554/eLife.46421 and PMC identifier 6609332.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Research on neuropeptide function has advanced rapidly, yet there is still no spatio-temporally resolved method to measure the release of neuropeptides in vivo. Here we introduce Neuropeptide Release Reporters (NPRRs): novel genetically-encoded sensors with high temporal resolution and genetic specificity. Using the <i>Drosophila</i> larval neuromuscular junction (NMJ) as a model, we provide evidence that NPRRs recapitulate the trafficking and packaging of native neuropeptides, and report stimulation-evoked neuropeptide release events as real-time changes in fluorescence intensity, with sub-second temporal resolution.
Medical subject headings
- Genes, Reporter
- Genetic Engineering
- Molecular Imaging
- Neuropeptides
- Synapses