Best practices and benchmarks for intact protein analysis for top-down mass spectrometry.
review · Level V
Where this comes from
- Record sourced from PubMed, PMID 31249407.
- Also identified by DOI 10.1038/s41592-019-0457-0 and PMC identifier 6719561.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.
Medical subject headings
- Benchmarking
- Mass Spectrometry
- Proteins