Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues.
other · Level V
Where this comes from
- Record sourced from PubMed, PMID 31273194.
- Also identified by DOI 10.1038/s41467-019-10534-0 and PMC identifier 6609674.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of clearing protocols, which will facilitate wide adoption by preclinical researchers and clinical laboratories. In particular, the open-top geometry provides unsurpassed versatility to interface with a wide range of accessory technologies in the future.
Medical subject headings
- Microscopy, Fluorescence