MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31294691.
- Also identified by DOI 10.7554/eLife.45696 and PMC identifier 6697447.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The non-destructive collection of ultrathin sections on silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable. In MagC introduced here, sample blocks are augmented with a magnetic resin enabling the remote actuation and collection of hundreds of sections on wafer. MagC allowed the correlative visualization of neuroanatomical tracers within their ultrastructural volumetric electron microscopy context.
Medical subject headings
- Histocytological Preparation Techniques
- Microscopy
- Microscopy, Electron