A genetically encodable cell-type-specific protein synthesis inhibitor.

Heumüller, Maximilian; Glock, Caspar; Rangaraju, Vidhya; Biever, Anne; Schuman, Erin M · Nat Methods · 2019

basic_science · Level V

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Abstract

Chemical inhibitors have revealed requirements for protein synthesis that drive cellular plasticity. We developed a genetically encodable protein synthesis inhibitor (gePSI) to achieve cell-type-specific temporal control of protein synthesis. Controlled expression of the gePSI in neurons or glia resulted in rapid, potent and reversible cell-autonomous inhibition of protein synthesis. Moreover, gePSI expression in a single neuron blocked the structural plasticity induced by single-synapse stimulation.

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