Monitoring of Cell Layer Integrity with a Current-Driven Organic Electrochemical Transistor.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31318183.
- Also identified by DOI 10.1002/adhm.201900128.
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Abstract
The integrity of CaCo-2 cell barriers is investigated by organic electrochemical transistors (OECTs) in a current-driven configuration. Ion transport through cellular barriers via the paracellular pathway is modulated by tight junctions between adjacent cells. Rupturing its integrity by H<sub>2</sub> O<sub>2</sub> is monitored by the change of the output voltage in the transfer characteristics. It is demonstrated that by operating the OECT in a current-driven configuration, the sensitive and temporal resolution for monitoring the cell barrier integrity is strongly enhanced as compared to the OECT transient response measurement. As a result, current-driven OECTs are useful tools to assess dynamic and critical changes in tight junctions, relevant for clinical applications as drug targeting and screening.
Medical subject headings
- Electrochemistry
- Transistors, Electronic