The regulatory B cell-mediated peripheral tolerance maintained by mast cell IL-5 suppresses oxazolone-induced contact hypersensitivity.

Kim, Hyuk Soon; Lee, Min Bum; Lee, Dajeong; Min, Keun Young; Koo, Jimo; Kim, Hyun Woo; Park, Young Hwan; Kim, Su Jeong et al. · Sci Adv · 2019

basic_science · Level V

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Abstract

The function of regulatory immune cells in peripheral tissues is crucial to the onset and severity of various diseases. Interleukin-10 (IL-10)-producing regulatory B (IL-10<sup>+</sup> B<sub>reg</sub>) cells are known to suppress various inflammatory diseases. However, evidence for the mechanism by which IL-10<sup>+</sup> B<sub>reg</sub> cells are generated and maintained is still very limited. Here, we found that IL-10<sup>+</sup> B<sub>reg</sub> cells suppress the activation of IL-13-producing type 2 innate lymphoid cells (IL-13<sup>+</sup> ILC2s) in an IL-10-dependent manner in mice with oxazolone-induced severe contact hypersensitivity (CHS). Mast cell (MC) IL-5 was important for maintaining the population of IL-10<sup>+</sup> B<sub>reg</sub> cells in peripheral lymphoid tissues. Overall, these results uncover a previously unknown mechanism of MCs as a type of immunoregulatory cell and elucidate the cross-talk among MCs, IL-10<sup>+</sup> B<sub>reg</sub> cells, and IL-13<sup>+</sup> ILC2s in CHS.

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