Optical/MRI dual-modality imaging of M1 macrophage polarization in atherosclerotic plaque with MARCO-targeted upconversion luminescence probe.
basic_science · Level V
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- Record sourced from PubMed, PMID 31382209.
- Also identified by DOI 10.1016/j.biomaterials.2019.119378.
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Abstract
Pro-inflammatory M1 macrophage is identified as a prominent component initializing the progress of vulnerable atherosclerotic plaque. Here, we constructed anti-MARCO NaGdF<sub>4</sub>:Yb,Er@NaGdF<sub>4</sub> upconversion nanoparticles (UCNPs) by conjugating polyclonal MARCO antibody to the surface of NaGdF<sub>4</sub>:Yb,Er@NaGdF<sub>4</sub>via condensation reaction. UCNPs displayed highly mono-dispersion with average sizes of 26.7 ± 0.8 nm and favorable biocompatibility. In vivo upconversion optical imaging revealed that distinctive fluorescence signal could be observed in the regions of carotid artery 10 min post-injection, reached peak value at 1 h and decreased back to baseline at 24 h post-injection. The carotid artery wall demonstrated high signal intensity on T1-weighted MR images after anti-MARCO UCNPs injection, as determined by 7.0T MRI. Immunofluorescence staining of tissue section of carotid artery revealed that MARCO was highly abundant in shoulder regions of plaque. Anti-MARCO UCNPs is a promising optical/MRI dual-modality imaging probe which can non-invasively reflect M1 phenotype macrophages behavior in vivo.
Medical subject headings
- Cell Polarity
- Luminescence
- Macrophages
- Magnetic Resonance Imaging
- Multimodal Imaging
- Optical Imaging
- Plaque, Atherosclerotic
- Receptors, Immunologic