Structure elucidation of colibactin and its DNA cross-links.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31395743.
- Also identified by DOI 10.1126/science.aax2685 and PMC identifier 6820679.
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Abstract
Colibactin is a complex secondary metabolite produced by some genotoxic gut <i>Escherichia coli</i> strains. The presence of colibactin-producing bacteria correlates with the frequency and severity of colorectal cancer in humans. However, because colibactin has not been isolated or structurally characterized, studying the physiological effects of colibactin-producing bacteria in the human gut has been difficult. We used a combination of genetics, isotope labeling, tandem mass spectrometry, and chemical synthesis to deduce the structure of colibactin. Our structural assignment accounts for all known biosynthetic and cell biology data and suggests roles for the final unaccounted enzymes in the colibactin gene cluster.
Medical subject headings
- DNA Adducts
- Peptides
- Polyketides