Expanding C-T base editing toolkit with diversified cytidine deaminases.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31399578.
- Also identified by DOI 10.1038/s41467-019-11562-6 and PMC identifier 6689024.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Base editing tools for cytosine to thymine (C-T) conversion enable genome manipulation at single base-pair resolution with high efficiency. Available base editors (BEs) for C-T conversion (CBEs) have restricted editing scopes and nonnegligible off-target effects, which limit their applications. Here, by screening diversified lamprey cytidine deaminases, we establish various CBEs with expanded and diversified editing scopes, which could be further refined by various fusing strategies, fusing at either N-terminus or C-terminus of nCas9. Furthermore, off-target analysis reveals that several CBEs display improved fidelity. Our study expands the toolkits for C-T conversion, serves as guidance for appropriate choice and offers a framework for benchmarking future improvement of base editing tools.
Medical subject headings
- Cytidine Deaminase
- Cytosine
- Gene Editing
- Thymine