Oblique-plane single-molecule localization microscopy for tissues and small intact animals.
basic_science · Level V
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- Record sourced from PubMed, PMID 31427757.
- Also identified by DOI 10.1038/s41592-019-0510-z.
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Abstract
Single-molecule localization microscopy (SMLM), while well established for cultured cells, is not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique-plane microscopy (obSTORM), which by directly imaging oblique sections of samples with oblique light-sheet illumination offers a deep and volumetric SMLM platform that is convenient for standard tissue samples and small intact animals. We demonstrate super-resolution imaging at depths of up to 66 µm for cells, Caenorhabditis elegans gonads, Drosophila melanogaster larval brain, mouse retina and brain sections, and whole stickleback fish.
Medical subject headings
- Brain
- Caenorhabditis elegans
- Drosophila melanogaster
- Fishes
- Microscopy, Fluorescence
- Retina
- Single Molecule Imaging