An efficient auxin-inducible degron system with low basal degradation in human cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 31451765.
- Also identified by DOI 10.1038/s41592-019-0512-x.
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Abstract
Auxin-inducible degron technology allows rapid and controlled protein depletion. However, basal degradation without auxin and inefficient auxin-inducible depletion have limited its utility. We have identified a potent auxin-inducible degron system composed of auxin receptor F-box protein AtAFB2 and short degron miniIAA7. The system showed minimal basal degradation and enabled rapid auxin-inducible depletion of endogenous human transmembrane, cytoplasmic and nuclear proteins in 1 h with robust functional phenotypes.
Medical subject headings
- Arabidopsis Proteins
- Carcinoma, Squamous Cell
- Indoleacetic Acids
- Proteolysis
- Receptors, Cell Surface
- Skin Neoplasms
- Transcription Factors