Live imaging of mRNA using RNA-stabilized fluorogenic proteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31471614.
- Also identified by DOI 10.1038/s41592-019-0531-7 and PMC identifier 6719798.
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Abstract
Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA-protein complexes that enable live imaging of mRNA in living cells.
Medical subject headings
- Aptamers, Nucleotide
- Fluorescence
- Fluorescent Dyes
- Microscopy, Fluorescence
- Molecular Imaging
- RNA, Messenger