Immunogenicity of a rheumatoid arthritis protective sequence when acquired through microchimerism.

Abstract

HLA class II genes provide the strongest genetic contribution to rheumatoid arthritis (RA). <i>HLA-DRB1</i> alleles encoding the sequence DERAA are RA-protective. Paradoxically, RA risk is increased in women with DERAA⁺ children born prior to onset. We developed a sensitive qPCR assay specific for DERAA, and found 53% of DERAA^-/- women with RA had microchimerism (Mc; pregnancy-derived allogeneic cells) carrying DERAA (DERAA-Mc) vs. 6% of healthy women. DERAA-Mc quantities correlated with an RA-risk genetic background including DERAA-binding <i>HLA-DQ</i> alleles, early RA onset, and aspects of RA severity. CD4⁺ T cells showed stronger response against DERAA⁺ vs. DERAA^- allogeneic cell lines in vitro, in line with an immunogenic role of allogeneic DERAA. Results indicate a model where DERAA-Mc activates DERAA-directed T cells that are naturally present in DERAA^-/- individuals and can have cross-reactivity against joint antigens. Moreover, we provide an explanation for the enigmatic observation that the same HLA sequence differentially affects RA risk through Mendelian inheritance vs. microchimeric cell acquisition.