High-definition spatial transcriptomics for in situ tissue profiling.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31501547.
- Also identified by DOI 10.1038/s41592-019-0548-y and PMC identifier 6765407.
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Abstract
Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.
Medical subject headings
- Gene Expression Profiling
- Transcriptome