Molecular resolution imaging by repetitive optical selective exposure.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31501551.
- Also identified by DOI 10.1038/s41592-019-0544-2.
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Abstract
We introduce an interferometric single-molecule localization method for super-resolution fluorescence microscopy. Fluorescence molecules are located by the intensities of multiple excitation patterns of an interference fringe, providing around a twofold improvement in the localization precision compared with the conventional imaging with the same photon budget. We demonstrate this technique by resolving nanostructures down to 5 nm in size over a large 25 × 25 μm<sup>2</sup> field of view.
Medical subject headings
- Microscopy, Fluorescence
- Single Molecule Imaging