Liquid application method for time-resolved analyses by serial synchrotron crystallography.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31527838.
- Also identified by DOI 10.1038/s41592-019-0553-1.
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Abstract
We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.
Medical subject headings
- Crystallography
- Synchrotrons