Adenine base editors catalyze cytosine conversions in human cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31548727.
- Also identified by DOI 10.1038/s41587-019-0254-4.
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Abstract
Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5-7) and in a confined TC*N sequence context. Adenine base editor-induced cytosine substitutions occur independently of adenosine conversions with an efficiency of up to 11.2% and reduce the number of suitable targeting sites for high-specificity base editing.
Medical subject headings
- Aminohydrolases
- CRISPR-Associated Protein 9
- Deoxyribonuclease I
- Gene Editing