A tissue-like platform for studying engineered quiescent human T-cells' interactions with dendritic cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31552826.
- Also identified by DOI 10.7554/eLife.48221 and PMC identifier 6910819.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Research in the field of human immunology is restricted by the lack of a system that reconstitutes the <i>in-situ</i>activation dynamics of quiescent human antigen-specific T-cells interacting with dendritic cells. Here we report a tissue-like system that recapitulates the dynamics of engineered primary human immune cell. Our approach facilitates real-time single-cell manipulations, tracking of interactions and functional responses complemented by population-based measurements of cytokines, activation status and proliferation. As a proof of concept, we recapitulate immunological phenomenon such as CD4 T-cells' help to CD8 T-cells through enhanced maturation of DCs and the effect of PD-1 checkpoint blockades. In addition, we characterise unique dynamics of T-cell/DC interactions as a function of antigen affinity.
Medical subject headings
- CD4-Positive T-Lymphocytes
- CD8-Positive T-Lymphocytes
- Cell Communication
- Dendritic Cells
- Lymphocyte Activation