Changes in the gut microbiota during and after commercial helium-oxygen saturation diving in China.

Abstract

The influence of commercial helium-oxygen saturation diving on divers' gut microbiotas was assessed to provide dietary suggestion. Faecal samples of 47 divers working offshore were collected before (T1), during (T2) and after (T3) saturation diving. Their living and excursion depths were 55-134 metres underwater with a saturation duration of 12-31 days and PaO₂ of 38-65 kPa. The faecal samples were examined through 16S ribosomal DNA amplicon sequencing based on the Illumina sequencing platform to analyse changes in the bacteria composition in the divers' guts. Although the α and β diversity of the gut microbiota did not change significantly, we found that living in a hyperbaric environment of helium-oxygen saturation decreased the abundance of the genus <i>Bifidobacterium</i>, an obligate anaerobe, from 2.43%±3.83% at T1 to 0.79%±1.23% at T2 and 0.59%±0.79% at T3. Additionally, the abundance of some short-chain fatty acid (SCFA)-producing bacteria, such as <i>Fusicatenibacter, Faecalibacterium</i>, rectale group and <i>Anaerostipes</i>, showed a decreased trend in the order of before, during and after diving. On the contrary, the abundance of species, such as <i>Lactococcus garvieae</i>, <i>Actinomyces odontolyticus</i>, <i>Peptoclostridium difficile</i>, <i>Butyricimonas virosa</i>, <i>Streptococcus mutans</i>, <i>Porphyromonas asaccharolytica</i> and <i>A. graevenitzii</i>, showed an increasing trend, but most of them were pathogens. Occupational exposure to high pressure in a helium-oxygen saturation environment decreased the abundance of <i>Bifidobacterium</i> and some SCFA-producing bacteria, and increased the risk of pathogenic bacterial infection. Supplementation of the diver diet with probiotics or prebiotics during saturation diving might prevent these undesirable changes.