Preparation and characteristics of electrospinning PTH-Fc/PLCL/SF membranes for bioengineering applications.

Abstract

Alveolar bone loss is always a big challenge for oral implant placement. Guided bone regeneration (GBR) is one of the solutions to fix this problem. Among all the factors, the barrier membrane plays a key role in this technology. At present, lack of the osteoinductivity remains the biggest weakness for clinical application. Focusing on this, we tried to fabricate an osteoinductive three-dimensional (3D) nanofiber membrane with drug releasing system by introducing parathyroid hormone (PTH)-Fc into the poly(lactic acid-co-caprolactone) PLCL/SF solution for electrospinning. The characteristics were determined by scanning electron microscopy, X-ray diffraction, contact angle measurement, and mechanical strength. According to the optimum parameters tested, we proceed to the in vitro experiments. Drug release and matrix degradation profiles of electrospinning fibers were investigated. Cell morphology was observed and cell proliferation was analyzed after bone marrow mesenchymal stem cells (BMMSCs) were seeded. The results showed favorable physical and chemical properties and good biocompatibility of these 3D nanofibers. The effect on the BMMSCs suggested the drug releasing promoted osteogenic differentiation of cells stably. This study exhibited a promising method of GBR oeteoinductive membrane fabrication. The bone reconstruction will be benefited from the osteogenic differentiation positive drug releasing system.