Photoactivation of silicon rhodamines via a light-induced protonation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31594948.
- Also identified by DOI 10.1038/s41467-019-12480-3 and PMC identifier 6783549.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore's outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.
Medical subject headings
- Fluorescent Dyes
- Intravital Microscopy
- Rhodamines
- Silicon
- Single Molecule Imaging