Acetyl-CoA-mediated activation of Mycobacterium tuberculosis isocitrate lyase 2.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31604954.
- Also identified by DOI 10.1038/s41467-019-12614-7 and PMC identifier 6788997.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Isocitrate lyase is important for lipid utilisation by Mycobacterium tuberculosis but its ICL2 isoform is poorly understood. Here we report that binding of the lipid metabolites acetyl-CoA or propionyl-CoA to ICL2 induces a striking structural rearrangement, substantially increasing isocitrate lyase and methylisocitrate lyase activities. Thus, ICL2 plays a pivotal role regulating carbon flux between the tricarboxylic acid (TCA) cycle, glyoxylate shunt and methylcitrate cycle at high lipid concentrations, a mechanism essential for bacterial growth and virulence.
Medical subject headings
- Acetyl Coenzyme A
- Isocitrate Lyase
- Mycobacterium tuberculosis