Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31611691.
- Also identified by DOI 10.1038/s41592-019-0592-7.
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Abstract
Intracellular antibodies have become powerful tools for imaging, modulating and neutralizing endogenous target proteins. Here, we describe an optogenetically activated intracellular antibody (optobody) consisting of split antibody fragments and blue-light inducible heterodimerization domains. We expanded this optobody platform by generating several optobodies from previously developed intracellular antibodies, and demonstrated that photoactivation of gelsolin and β2-adrenergic receptor (β2AR) optobodies suppressed endogenous gelsolin activity and β2AR signaling, respectively.
Medical subject headings
- Antibodies
- Gelsolin
- Optogenetics
- Receptors, Adrenergic, beta-2