mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31611693.
- Also identified by DOI 10.1038/s41592-019-0613-6.
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Abstract
Super-resolution correlative light and electron microscopy (SR-CLEM) is a powerful approach for imaging specific molecules at the nanoscale in the context of the cellular ultrastructure. Epon epoxy resin embedding offers advantages for SR-CLEM, including ultrastructural preservation and high quality sectioning. However, Epon embedding eliminates fluorescence from most fluorescent proteins. We describe a photocontrollable fluorescent protein, mEosEM, that can survive Epon embedding after osmium tetroxide (OsO<sub>4</sub>) treatment for improved SR-CLEM.
Medical subject headings
- Epoxy Resins
- Luminescent Proteins
- Microscopy, Electron
- Organelles
- Osmium Tetroxide
- Specimen Handling