Origami-paper-based device for microvesicle/exosome preconcentration and isolation.

Kim, Hyerin; Lee, Kyu Hyoung; Han, Sung Il; Lee, Dongho; Chung, Seok; Lee, Dohwan; Lee, Jeong Hoon · Lab Chip · 2019

basic_science · Level V

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Abstract

Microvesicles and exosomes are promising liquid biopsy biomarkers. However, conventional isolation techniques damage and contaminate the biomarkers. We developed an origami-paper-based device for effective isolation of biomarkers with less damage and in fewer steps. The multi-folded device enables the preconcentration of the microvesicles/exosomes on specific layers (∼5-fold) by the ion concentration polarization technique and they were simply isolated from the rest of the sample by unfolding the device.

Medical subject headings