Kilohertz two-photon brain imaging in awake mice.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31659327.
- Also identified by DOI 10.1038/s41592-019-0597-2 and PMC identifier 9438750.
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Abstract
Two-photon microscopy is a mainstay technique for imaging in scattering media and normally provides frame-acquisition rates of ~10-30 Hz. To track high-speed phenomena, we created a two-photon microscope with 400 illumination beams that collectively sample 95,000-211,000 µm<sup>2</sup> areas at rates up to 1 kHz. Using this microscope, we visualized microcirculatory flow, fast venous constrictions and neuronal Ca<sup>2+</sup> spiking with millisecond-scale timing resolution in the brains of awake mice.
Medical subject headings
- Brain
- Microscopy, Fluorescence, Multiphoton