Non-reversible tissue fixation retains extracellular vesicles for in situ imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31712780.
- Also identified by DOI 10.1038/s41592-019-0623-4 and PMC identifier 9344330.
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Abstract
Extracellular vesicles (EVs) are secreted nanosized particles with many biological functions and pathological associations. The inability to image EVs in fixed tissues has been a major limitation to understanding their role in healthy and diseased tissue microenvironments. Here, we show that crosslinking mammalian tissues with formaldehyde results in significant EV loss, which can be prevented by additional fixation with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) for visualization of EVs in a range of normal and cancer tissues.
Medical subject headings
- Extracellular Vesicles
- Tissue Fixation