Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31719538.
- Also identified by DOI 10.1038/s41467-019-13152-y and PMC identifier 6851099.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Transcription by RNA polymerase (RNAP) is coupled with translation in bacteria. Here, we observe the dynamics of transcription and subcellular localization of a specific gene locus (encoding a non-membrane protein) in living E. coli cells at subdiffraction-limit resolution. The movement of the gene locus to the nucleoid periphery correlates with transcription, driven by either E. coli RNAP or T7 RNAP, and the effect is potentiated by translation.
Medical subject headings
- Cell Nucleus
- Escherichia coli
- Genetic Loci
- Protein Biosynthesis
- Transcription, Genetic