SpCas9 activity prediction by DeepSpCas9, a deep learning-based model with high generalization performance.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31723604.
- Also identified by DOI 10.1126/sciadv.aax9249 and PMC identifier 6834390.
- Licence recorded as CC BY-NC.
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Abstract
We evaluated SpCas9 activities at 12,832 target sequences using a high-throughput approach based on a human cell library containing single-guide RNA-encoding and target sequence pairs. Deep learning-based training on this large dataset of SpCas9-induced indel frequencies led to the development of a SpCas9 activity-predicting model named DeepSpCas9. When tested against independently generated datasets (our own and those published by other groups), DeepSpCas9 showed high generalization performance. DeepSpCas9 is available at http://deepcrispr.info/DeepSpCas9.
Medical subject headings
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- Deep Learning