Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31740821.
- Also identified by DOI 10.1038/s41592-019-0630-5 and PMC identifier 6949126.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Spatially controlled cell adhesion on electron microscopy supports remains a bottleneck in specimen preparation for cellular cryo-electron tomography. Here, we describe contactless and mask-free photo-micropatterning of electron microscopy grids for site-specific deposition of extracellular matrix-related proteins. We attained refined cell positioning for micromachining by cryo-focused ion beam milling. Complex micropatterns generated predictable intracellular organization, allowing direct correlation between cell architecture and in-cell three-dimensional structural characterization of the underlying molecular machinery.
Medical subject headings
- Cell Membrane
- Cryoelectron Microscopy
- Green Fluorescent Proteins
- Image Processing, Computer-Assisted
- Molecular Imaging
- Specimen Handling