Combining dielectrophoresis and computer vision for precise and fully automated single-cell handling and analysis.

Godino, Neus; Pfisterer, Felix; Gerling, Tobias; Guernth-Marschner, Christian; Duschl, Claus; Kirschbaum, Michael · Lab Chip · 2019

basic_science · Level V

Where this comes from

Abstract

With the advent of single-cell technologies comes the necessity for efficient protocols to process single cells. We combine dielectrophoresis with open source computer vision programming to automatically control the trajectories of single cells inside a microfluidic device. Using real-time image analysis, individual cells are automatically selected, isolated and spatially arranged.

Medical subject headings