Simultaneous multi-signal quantification for highly precise serodiagnosis utilizing a rationally constructed platform.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31767865.
- Also identified by DOI 10.1038/s41467-019-13358-0 and PMC identifier 6877524.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Serodiagnosis with a single quantification method suffers from high false positive/negative rates. In this study, a three-channel platform with an accessional instrumented system was constructed for simultaneous electrochemical, luminescent, and photothermal quantification of H<sub>2</sub>S, a bio-indicator for acute pancreatitis (AP) diagnosis. Utilizing the specific reaction between platform and H<sub>2</sub>S, the three-channel platform showed high sensitivity and selectivity in the biological H<sub>2</sub>S concentration range. The three-channel platform was also feasible for identifying the difference in the plasma H<sub>2</sub>S concentrations of AP and normal mice. More importantly, the precision of AP serodiagnosis was significantly improved (>99.0%) using the three-signal method based on the three-channel platform and an optimized threshold, which was clearly higher than that of the single- or two-signal methods (79.5%-94.1%). Our study highlights the importance of constructing a multichannel platform for the simultaneous multi-signal quantification of bio-indicators, and provides rigorous ways to improve the precision of medical serodiagnosis.
Medical subject headings
- Electrochemical Techniques
- Hydrogen Sulfide
- Luminescent Measurements
- Pancreatitis
- Serologic Tests