DIA-NN: neural networks and interference correction enable deep proteome coverage in high throughput.

Demichev, Vadim; Messner, Christoph B; Vernardis, Spyros I; Lilley, Kathryn S; Ralser, Markus · Nat Methods · 2020

basic_science · Level V

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Abstract

We present an easy-to-use integrated software suite, DIA-NN, that exploits deep neural networks and new quantification and signal correction strategies for the processing of data-independent acquisition (DIA) proteomics experiments. DIA-NN improves the identification and quantification performance in conventional DIA proteomic applications, and is particularly beneficial for high-throughput applications, as it is fast and enables deep and confident proteome coverage when used in combination with fast chromatographic methods.

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