Localization microscopy at doubled precision with patterned illumination.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31819263.
- Also identified by DOI 10.1038/s41592-019-0657-7 and PMC identifier 6989044.
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Abstract
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.
Medical subject headings
- DNA
- Lighting
- Microscopy, Fluorescence
- Models, Theoretical
- Nanostructures
- Single Molecule Imaging