Magnetic resonance measurements of cellular and sub-cellular membrane structures in live and fixed neural tissue.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31829935.
- Also identified by DOI 10.7554/eLife.51101 and PMC identifier 6977971.
- Licence recorded as CC0.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We develop magnetic resonance (MR) methods for real-time measurement of tissue microstructure and membrane permeability of live and fixed excised neonatal mouse spinal cords. Diffusion and exchange MR measurements are performed using the strong static gradient produced by a single-sided permanent magnet. Using tissue delipidation methods, we show that water diffusion is restricted solely by lipid membranes. Most of the diffusion signal can be assigned to water in tissue which is far from membranes. The remaining 25% can be assigned to water restricted on length scales of roughly a micron or less, near or within membrane structures at the cellular, organelle, and vesicle levels. Diffusion exchange spectroscopy measures water exchanging between membrane structures and free environments at 100 s<sup>-1</sup>.
Medical subject headings
- Cell Membrane
- Diffusion Magnetic Resonance Imaging
- Intracellular Membranes
- Magnetic Resonance Spectroscopy
- Spinal Cord