Classification of mouse B cell types using surfaceome proteotype maps.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31844046.
- Also identified by DOI 10.1038/s41467-019-13418-5 and PMC identifier 6915781.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
System-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when samples are limited. Here, we miniaturize and automate the previously described Cell Surface Capture (CSC) technology, increasing sensitivity, reproducibility and throughput. We use this technology, which we call autoCSC, to create population-specific surfaceome maps of developing mouse B cells and use targeted flow cytometry to uncover developmental cell subpopulations.
Medical subject headings
- B-Lymphocyte Subsets
- Cell Differentiation
- High-Throughput Screening Assays
- Membrane Proteins
- Proteomics