Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31844291.
- Also identified by DOI 10.1038/s41587-019-0331-8 and PMC identifier 7775643.
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Abstract
Here we describe TRACE (T7 polymerase-driven continuous editing), a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA polymerase. TRACE induces high rates of mutagenesis over multiple cell generations in genes under the control of a T7 promoter integrated in the genome. We used TRACE in a MEK1 inhibitor-resistance screen, and identified functionally correlated mutations.
Medical subject headings
- DNA
- Gene Editing
- Mutagenesis