The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31855181.
- Also identified by DOI 10.7554/eLife.49784 and PMC identifier 6952179.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Previously we reported that a process called inter-fork strand annealing (IFSA) causes genomic deletions during the termination of DNA replication when an active replication fork converges on a collapsed fork (Morrow et al., 2017). We also identified the FANCM-related DNA helicase Fml1 as a potential suppressor of IFSA. Here, we confirm that Fml1 does indeed suppress IFSA, and show that this function depends on its catalytic activity and ability to interact with Mhf1-Mhf2 via its C-terminal domain. Finally, a plausible mechanism of IFSA suppression is demonstrated by the finding that Fml1 can catalyse regressed fork restoration in vitro.
Medical subject headings
- Chromosomal Proteins, Non-Histone
- DNA Helicases
- Recombination, Genetic
- Schizosaccharomyces pombe Proteins