Tunable light and drug induced depletion of target proteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31949141.
- Also identified by DOI 10.1038/s41467-019-14160-8 and PMC identifier 6965615.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Biological processes in development and disease are controlled by the abundance, localization and modification of cellular proteins. We have developed versatile tools based on recombinant E3 ubiquitin ligases that are controlled by light or drug induced heterodimerization for nanobody or DARPin targeted depletion of endogenous proteins in cells and organisms. We use this rapid, tunable and reversible protein depletion for functional studies of essential proteins like PCNA in DNA repair and to investigate the role of CED-3 in apoptosis during Caenorhabditis elegans development. These independent tools can be combined for spatial and temporal depletion of different sets of proteins, can help to distinguish immediate cellular responses from long-term adaptation effects and can facilitate the exploration of complex networks.
Medical subject headings
- Caenorhabditis elegans Proteins
- Cytological Techniques
- Light
- Ubiquitin-Protein Ligases