Neutrophil membrane-enveloped nanoparticles for the amelioration of renal ischemia-reperfusion injury in mice.

Abstract

Ischemia-reperfusion (I/R) injury initiates and exacerbates a series of oxidative and inflammatory events, and causes high morbidity and mortality. Despite the progress made with recent clinical use of anti-malarial drugs, the response rate of I/R injury treatment remains unsatisfactory. Here, we showed a neutrophil membrane-enveloped Coenzyme Q (N-NP_CoQ10) nanoparticle strategy for I/R injury treatment. We validated the physicochemical and biological reproducibility of the nanoparticles and tested the protective effects of N-NP_CoQ10 in oxygen-glucose deprivation/reperfusion model and renal I/R injury mouse model. N-NP_CoQ10 nanoparticles administration exhibited synergistic protective effect against I/R injury, which significantly reduced oxidative damage in vitro and in vivo, inhibited renal cell apoptosis, attenuated inflammatory response in renal I/R injury model, and finally improved renal function of I/R injury mice. The N-NP_CoQ10 nanoparticles administration provides an efficient way to deliver anti-oxidant that suppresses oxidative damages and neutralize proinflammatory cytokines during renal I/R injury, which might be a potential strategy for renal acute kidney injury treatment. STATEMENT OF SIGNIFICANCE: The neutrophil membrane-enveloped Coenzyme Q nanoparticles (N-NP_CoQ10) provides an efficient way to protect oxidative, inflammatory, and apoptotic reaction in renal I/R injury, which might be a potential strategy for renal acute kidney injury treatment.