Imaging brain activity during complex social behaviors in Drosophila with Flyception2.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32001689.
- Also identified by DOI 10.1038/s41467-020-14487-7 and PMC identifier 6992788.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Optical in vivo recordings from freely walking Drosophila are currently possible only for limited behaviors. Here, we expand the range of accessible behaviors with a retroreflective marker-based tracking and ratiometric brain imaging system, permitting brain activity imaging even in copulating male flies. We discover that P1 neurons, active during courtship, are inactive during copulation, whereas GABAergic mAL neurons remain active during copulation, suggesting a countervailing role of mAL in opposing P1 activity during mating.
Medical subject headings
- Copulation
- Drosophila
- Neuroimaging
- Sexual Behavior, Animal
- Social Behavior