Surface protein imputation from single cell transcriptomes by deep neural networks.

Zhou, Zilu; Ye, Chengzhong; Wang, Jingshu; Zhang, Nancy R · Nat Commun · 2020

other · Level V

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Abstract

While single cell RNA sequencing (scRNA-seq) is invaluable for studying cell populations, cell-surface proteins are often integral markers of cellular function and serve as primary targets for therapeutic intervention. Here we propose a transfer learning framework, single cell Transcriptome to Protein prediction with deep neural network (cTP-net), to impute surface protein abundances from scRNA-seq data by learning from existing single-cell multi-omic resources.

Medical subject headings